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B.S., Northeastern University; Ph.D., Boston University; Helen Hay Whitney Postdoctoral Fellow, Stanford University.


Fundamental problems in developmental biology, such as the generation of asymmetry, differential transcription, and the execution of positional information, are all exhibited during the cell cycle of the bacterium, Caulobacter crescentus. Caulobacter undergoes a fixed sequence of differentiation events within each cell cycle. Cell division yields morphologically and biochemically dissimilar daughter cells; a swarmer cell which posesses a single polar flagellum and a non-motile stalked cell. During the latter portion of the DNA replication phase, the components of the flagellum are synthesized and assembled at the pole of the predivisional cell that is opposite the stalk.

For a subset of flagellar genes, positional information is generated through localized transcription in one pole of the cell. The newly replicated chromosomes in the predivisional cell, therefore differ in their program of transcription as well as the ability to initiate DNA replication. The overall goal of our research program is to define the mechanisms by which this differential, global programming of the Caulobacter chromosome is accomplished. Using both genetic and biochemical approaches we are determining the mechanisms of both temporal and spatial transcriptional activation of these locally expressed promoters.

We are also interested in the mechanisms that couple cellular morphogenesis to gene expression. Early flagellar assembly events are required for the transcription of genes encoding structures that are assembled later in the flagellum. These same events are also required for normal cell division. We are determining how factors that regulate gene expression and cell division monitor the assembly of the cellular structures.

   Representative Publications

Clarke, C.F., Rowat, A.C., J.W. Gober "Osmotic stress: Is CoQ a membrane stabilizer?" Nat Chem Biol. (2014) Mar 18;10(4):242-3.

White, C.L., J.W. Gober "MreB: pilot or passenger of cell wall synthesis?" Trends Microbiol. (2012) Feb;20(2):74-9.

Xu, Z., Dutton, R.J., J.W. Gober "Direct interaction of FliX and FlbD is required for their regulatory activity in Caulobacter crescentus" BMC Microbiol. (2011) May 2;11:89.

White, C.L., Kitich A., J.W. Gober "Positioning cell wall synthetic complexes by the bacterial morphogenetic proteins MreB and MreD" Mol Microbiol. (2010) May;76(3):616-33.

England, J.C., Perchuk, B.S., Laub, M.T., J.W. Gober, "Global regulation of gene expression and cell differentiation in Caulobacter crescentus in response to nutrient availability" J Bacteriol. (2010) Feb;192(3):819-33.

Divkaruni, A., Baida, C. White, C., and J.W. Gober "The cell shape proteins MreB and MreC control cell morphogenesis by positioning cell wall synthetic complexes" Mol. Microbiol. (2007) 66:174-188.

Dutton, R.J., Xu, Z., and J.W. Gober "Linking structural assembly to gene expression: a novel mechanism for regulating the activity of a sigma54 transcription factor" Mol. Microbiol. (2005) 58:743-757.

Muir, R.E., Easter, J., and J.W. Gober "The trans-acting flagellar regulatory proteins, FliX and FlbD, play a central role in linking flagellar biogenesis and cytokinesis in Caulobacter crescentus" Microbiol. (2005) 151:3699-3711.

Divakaruni, A.V., R.R. Ogorzalek Loo, Y. Xie, J.A. Loo, and J.W. Gober "The cell-shape protein MreC interacts with extracytoplasmic proteins including cell wall assembly complexes in Caulobacter crescentus" Proc. Natl. Acad. Sci. U.S.A. (2005) 102:18602-18607.

Evilevitch, A., J.W. Gober, M. Phillips, C.M. Knobler, and W.M. Gelbart "Measurements of DNA lengths remaining in a viral capsid after osmotically suppressed partial ejection" Biophys. J. (2005) 88:751-756.

Muir, R.E. and J.W. Gober "Role of Integration Host Factor (IHF) in the transcriptional activation of flagellar gene expression in Caulobacter crescentus" J. Bacteriol. (2005) 187:949-960.

England, J.C., and J.W. Gober "Role of core promoter sequences in the mechanism of swarmer cell-specific silencing of gyrB transcription in Caulobacter crescentus" BMC Microbiol. (2005) 5:25-37.

Llewellyn, M., Dutton, R.J., Easter, J., O'Donnol, and J.W. Gober "The conserved flaF gene has a critical role in coupling flagellin translation and secretion in Caulobacter crescentus" Mol. Microbiol. (2005) 57:1127-1142.

Muir, R.E. and J.W. Gober " Regulation of FlbD activity by flagellum assembly is accomplished through direct interaction with the trans-acting factor, FliX." Mol. Microbiol. (2004) 54:715-730.

Figge, R.M., Divakaruni, A.V. and Gober, J.W. "MreB, the cell shape-determining bacterial actin homolog, coordinates cell wall morphogenesis in Caulobacter crescentus" Mol Microbiol. (2004) 51:1321-1332.

Figge, R.M., Gober, J.W. "Cell shape, division and development: the 2002 American Society for Microbiology (ASM) conference on prokaryotic development" Mol Microbiol. (2003) 47:1475-83.

Figge, R.M., Easter, J, Gober J.W. "Productive interaction between the chromosome partitioning proteins, ParA and ParB, is required for the progression of the cell cycle in Caulobacter crescentus" Mol Microbiol. (2003) 47:1225-37.

Muir, R.E. and Gober, J. W. "Mutations in FlbD that Relieve Flagellum Assembly Alter the Temporal and Spatial Pattern of Developmental Transcription in Caulobacter crescentus" Mol. Microbiol. (2002) 43:597-616.

Draper,G.C. and Gober, J.W. "Bacterial Chromosome Segregation." Annu. Rev. Microbiol. 56 (2002):567-597.

Easter, J. and Gober,
J.W. "ParB-stimulated nucleotide exchange regulates a switch in functionally distinct ParA activities." Mol. Cell. 10 (2002):427-434.

England, J.C. and J.W. Gober, J.W. "Cell cycle control of cell morphogenesis in Caulobacter" Curr. Op. Microbiol. (2001) 4:674-680.

Boyd, C., and Gober, J.W. "Temporal regulation of the genes encoding the flagellar proximal rod in Caulobacter crescentus." J. Bacteriol.183.2 (2001): 725-735.

Muir, R.E., T. O'Brien, and Gober,J.W. "The Caulobacter crescentus flagellar gene, fliX, encodes a novel trans-acting factor that couples flagellar assembly to transcription." Mol. Microbiol. 39.6 ( 2001): 1623-1637.

Muir, R.E. and Gober, J.W. "Regulation of late flagellar gene transcription and cell division by flagellum assembly in Caulobacter crescentus." Mol. Microbiol. 41.1 (2001): 117-130.

Mohl, D.A., J. Easter and Gober, J.W. "The Chromosome Partitioning Protein, ParB, is Required for Cytokinesis in Caulobacter crescentus." Mol. Microbiol. (2001) Nov;42(3):741-55.

Anderson, P.E., and Gober, J.W. "FlbT, the post-transcriptional regulator of flagellin synthesis in Caulobacter crescentus, interacts with the 5' untranslated region of flagellin mRNA." Mol. Microbiol 38.1 (2000): 41-52.

Gober, J.W., and England, J.C. "Regulation of flagellum biosynthesis and motility in Caulobacter In 'Prokaryotic Development'." American Society for Microbiology Press, Eds. Y. Brun and L. Shimketts. Washington, DC: (2000) 319-339.

Mangan, E.K., J. Malakooti, A. Caballero, P.E. Anderson, B. Ely, and Gober, J.W.  "FlbT couples flagellum assembly to gene expression in Caulobacter crescentus." J.Bacteriol. 181.19 (1999): 6160-6170.

Wheeler, R.T., Gober, J.W., and Shapiro, L.  "Protein localization during the Caulobacter crescentus cell cycle." Curr. Op. Microbiol. 6 (1998): 636-642.

Marques, M.D.V., S.L. Gomes, and Gober, J.W.  "A gene coding for a putative sigma 54 activator is developmentally regulated in Caulobacter crescentus." J. Bacteriol. 179.17 (1997): 5502-5510.

Mohl, D.A. and Gober, J.W.  "Cell Cycle-Dependent Polar Localization of Chromosome Partitioning Proteins in Caulobacter crescentus." Cell 88.5 (1997): 675-684.

Stephens, C., C. Mohr, C. Boyd, J. Maddock, J. Gober, and Shapiro, L.  "Identification of the fliI and fliJ components of the Caulobacter flagellar type III protein secretion system." J. Bacteriol 179.17 (1997):5355-5365.

Wingrove, J.A. and Gober, J.W.  "Identification of an asymmetrically localized sensor histidine kinase responsible for temporally and spatially regulated transcription."  Science 274 (1996): 597-601.

Gober, J.W. and Wingrove, J.A.  "Generation of asymmetry during Caulobacter cell differentiation." Sem. Dev. Biol 6 (1995): 325-333.

Mangan,E.K., M. Bartamian and Gober, J.W. "A mutation that uncouples flagellum assembly from transcription alters the temporal pattern of flagellar gene expression in Caulobacter crescentus." J. Bacteriol 177.11(1995): 3176-3184.

Gober, J.W. and Marques, M. "Regulation of cellular differentiation in Caulobacter." Microbiol. Mol. Biol. Rev. 59.1 (1995): 31-47.

Marques, M.V., and Gober, J.W.  "Activation of a temporally regulated Caulobacter promoter by upstream and downstream sequence elements." Mol. Microbiol. 16 (1995): 279-289.

Gober, J.W., Boyd, C., Jarvis, M., Mangan,E.,Rizzo, M., and Wingrove, J.A. "Temporal and spatial regulation of fliP, and early flagellar gene of Caulobacter cresentus that is required for motility and normal cell division." J. Bacteriol 177.13 (1995): 3656-3667.

Avedissian, M., Lessing, D., Gober, J.W., Shapiro, L., and Gomes, S.L.  "Regulation of the Caulobacter cresecentus dnaKJ operon." J. Bacteriol 177.12 (1995): 3479-3484.

Wingrove, J.A., and Gober, J.W. "A sigma 54 transcriptional activator also functions as a pole-specific repressor in Caulobacter." Genes & Dev. 8 (1994): 1839-1852.

Rizzo, M.F., Shapiro, L., and Gober, J.W.  "Asymmetric expression of the gyrase B gene from the replication-competent chromosome in the Caulobacter crescentus predivisional cell." J. Bacteriol. 175.21 (1993): 6970-6981.

Wingrove, J.A., Mangan, E.K. and Gober, J.W. "Spatial and temporal phosphorylation of a transcriptional activator regulates pole-specific gene expression in Caulobacter." Genes & Dev. 7 (1993): 1979-1992.

   Contact Info

Department of Chemistry & Biochemistry
Young Hall 5072C
Box 951569 (post)
607 Charles E. Young Drive East (courier)
Los Angeles, CA 90095-1569
Phone: (310) 206-9449
Lab Phone: (310) 206-5684
Fax: (310) 206-4038
Email: jwg@chem.ucla.edu
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