Spectrofluorimeter-SPEX: Single photon counting measurements of fluorescence intensity. Equipped with polarizers for fluorescence anisotropy measurements.

Circular Dichroism Spectrophotometer-JASCO : For the determination of secondary structure content and monitoring structural changes in proteins and nucleic acids. The instrument is equipped with a heating/cooling water bath for monitoring conformational changes as a function of temperature.

UV/Visible spectrophotometer- Hewlett Packard: This will be used for routine measurements of absorbance spectra and simple concentration measurements. Diode Array detection permits rapid measurement of full spectra. Although this is standard laboratory equipment, it is needed for convenient use of other equipment within the core facility.

Analytical Ultracentrifuge-Beckman Model XL-A: For sedimentation velocity, sedimentation equilibrium and diffusion coefficient measurements. Provides estimations of molecular weight, shape and binding constants.

Precision Density Meter-Mettler/Paar DMA O2D: For accurate determination of solution densities and partial specific volume (useful for molecular weight determination).

Pharmacia Biacore X: For detecting molecular interactions and determination of equilibrium binding constants and binding and dissociation rate constants.

Differential Scanning Calorimeter-Calorimetry Sciences Corp. NANO: For obtaining thermodynamic parameters of thermally induced conformational changes.

Titration Calorimeter-Calorimetric Sciences Corporation Jet: For measuring thermodynamic parameters of molecular interactions.

Stopped-Flow Reaction Analyzer-Applied Photophysics: For monitoring of fast reactions (instrument dead time <1ms). Possesses both absorbance and fluorescence detection capabilities.

Protein Purification System-Perseptive Biosystems BioCad 700E: A versatile HPLC system capable of automatically testing a large array of purification methods on all chromatography media. A wide range of flow rates permits use on both analytical and preparative scale columns.

Phosphorimager-Molecular Dynamics 445 SI: Used for sensitive and quantitative measurement of radioactivity on gels or blots.

DNA synthesizer-Beckman Oligo 1000M: Oligonucleotide synthesis. Performs 3 simultaneous syntheses with short cycle times.

Liquid Scintillation Counters-Beckman LS8100 and LS3133P: Radioactivity quantification.

CCD Gel Documentation System: For recording gels and quantification of band intensities.

A Wyatt Technology Corp. Three-angle Light Scattering Detector: For measurement of molecular weights and oligomerization states of our proteins. It is used mostly as a chromatography detector, but can also be used in batch mode (direct injection).